Affinity-based enrichment of methylated DNA sequences with methyl-CpG binding domain proteins followed by next generation sequencing (MBD-seq) utilizing the MethylMiner™ Methylated DNA Enrichment kit has been shown to be a powerful alternative to MeDIP-seq and the whole methylome sequencing technology of BS-seq,. Several high throughput profiling techniques (MeDIP-seq, ,, MIRA-seq, MBD-seq, MethylCap-seq, MethylC-seq, , BS-seq ) have been developed to study genome-wide methylation patterns. Recently, in addition to protein–DNA interactions, massively parallel sequencing has been used to identify open chromatin, histone modifications, , and DNA methylation.ĭNA methylation is one of the major epigenetic mechanisms that play an important role in a variety of cancers. ChIP-sequencing (ChIP-seq) has become a leading technology to interrogate in vivo protein-DNA interactions. The advance of next generation sequencing technology has revolutionized the research field of transcriptional regulation and systems biology,. This does not alter the authors' adherence to all the PLoS ONE policies on sharing data and materials.

The others are from OSU and have no roles at Life Tech. Meredith are employed at Life Technologies. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors CA, ML, MD, DK, G. XL is awarded a Howard Hughes Medical Institute Med Into Grad Fellowship. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.įunding: The study is funded by Biomedical Informatics Start-up funds. Received: JanuAccepted: JPublished: July 11, 2011Ĭopyright: © 2011 Lan et al. PLoS ONE 6(7):Įditor: Raja Jothi, National Institutes of Health, United States of America (2011) High Resolution Detection and Analysis of CpG Dinucleotides Methylation Using MBD-Seq Technology. Citation: Lan X, Adams C, Landers M, Dudas M, Krissinger D, Marnellos G, et al.

Resolution by Kim Loraine